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Department of Chemistry
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Energetics of Z-DNA binding protein-mediated helicity reversals in DNA, RNA, and DNA-RNA duplexes
- POSTED DATE : 2014-02-17
- WRITER :
- HIT : 3183
- Researcher : Bae S, Kim YG*
Z-DNA binding proteins (ZBPs) specifically recognize and stabilize lefthanded
double helices, including Z-DNA and Z-RNA. However, the energetics of Zform
stabilization by ZBPs have never been characterized due to the technical
limitations of bulk studies, resulting in an unclear understanding of the ZBP operational
mechanism at the molecular level. Here, we use single-molecule fluorescence resonance
energy transfer (FRET) to determine the energetics of Z-form stabilization by ZBP for
DNA, RNA, and DNA−RNA duplexes, revealing that the formation of B−Z or A−Z
junctions dominates the thermodynamics and kinetics of Z-form stabilization.
Furthermore, in contrast to general assumptions, the Z-form is most efficiently and
most rapidly formed in the DNA−RNA hybrid duplex due to the greatly reduced junction energy in the DNA−RNA hybrid.
double helices, including Z-DNA and Z-RNA. However, the energetics of Zform
stabilization by ZBPs have never been characterized due to the technical
limitations of bulk studies, resulting in an unclear understanding of the ZBP operational
mechanism at the molecular level. Here, we use single-molecule fluorescence resonance
energy transfer (FRET) to determine the energetics of Z-form stabilization by ZBP for
DNA, RNA, and DNA−RNA duplexes, revealing that the formation of B−Z or A−Z
junctions dominates the thermodynamics and kinetics of Z-form stabilization.
Furthermore, in contrast to general assumptions, the Z-form is most efficiently and
most rapidly formed in the DNA−RNA hybrid duplex due to the greatly reduced junction energy in the DNA−RNA hybrid.
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