High-affinity and controllable host-guest chemistry as a new supramolecular tool for biology and biomedicine

  • POSTED DATE : 2020-10-14
  • WRITER : 화학과
  • HIT : 55
  • DATE : 2020년 10월 15일(목) 오후 4시 30분
  • PLACE : Webex

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제  목 : High-affinity and controllable host-guest chemistry as a new supramolecular tool for biology and biomedicine
연  사 : 박경민 박사(IBS)
일  시 : 2020년 10월 15일(목) 오후 4시 30분


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-미팅룸 번호 : 571 508 642

High-affinity and controllable host-guest chemistry as a new supramolecular tool for biology and biomedicine

Kyeng Min Park

Center for Self-assembly and Complexity (CSC), Institute for Basic Science (IBS),
Pohang 37673, Republic of Korea

Due to a high binding affinity (Ka ~ 1013 M-1), the streptavidin (Sv) and biotin (BT) as a protein-ligand binding pair has been utilized as a useful tool for various bioapplications including protein imaging and identification. However, the Sv-BT pair has some intrinsic shortcomings; 1) interference of binding from endogenous biotins, 2) false positive signals caused by endogenously biotinylated proteins 3) difficulty in chemical modification and instability of streptavidin (protein) degraded by proteases in cellular conditions. Recently, we developed a new ultrastable and controllable synthetic binding pair as “supramolecular latching system” consisting of a pumpkin-shaped synthetic host molecule, cucurbit[7]uril (CB[7]) and adamantylamine (AdA), which has almost comparable (or even higher) binding affinity (Ka ~1012-15 M-1) to Sv-BT with unique features including 1) bio-orthogonality in binding which does not affected by endogenous biomolecules such as biotin, 2) small size with stable and robust chemical structure, 3) scalability using known chemical synthetic methods and 4) controllable binding affinity by treating strong competitors. In this talk, I will introduce our recent efforts to develop this supramolecular latching system as a new molecular tool for various bioapplications (Fig. 1) including visualization and identification of spatial proteins in cells and cost-effective purification of therapeutic proteins in high purity. In addition, future direction of research and development of this new molecular tool will be discussed.


Figure 1. Schematic illustration of “supramolecular latching system” and its bioapplications


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3. Kim, K. L.; Sung, G..; Sim, J.; Murray, J.; Li, M.; Lee, A.; Shrinidhi, A.; Park, K. M. M.*; Kim, K.*
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imaging” Nat. Commun. 2018 , 9, 1712.
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S.; Kim, K.* “Mono allyloxylated cucurbit[7]uril acts as an unconventional amphiphile to form
light responsive vesicles” Angew. Chem. Int. Ed. 2018 , 57, 3132