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제  목 : PERFORMANCE OF AMINE-RICH PLASMA POLYMERS IN BIOSENSING AND TISSUE ENGINEERING

연  사 : Prof. Lenka Zajickova(Central European Institute of Technology - CEITEC, Masaryk University, Brno, Czech Republic/Faculty of Science, Masaryk University, Brno, Czech Republic)
 

일  시 : 2014년 8월 14일 (목) 오후 4시 30분

장  소 : 화학관 세미나실 (330226호실)

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pERFORMANCE OF AMINE-RICH PLASMA POLYMERS IN BIOSENSING AND TISSUE ENGINEERING

Lenka Zajíčková^1,2, Anton Manakhov¹, Marek Eliáš^1,2, Petr Skládal^1,2, David Nečas^1,2,

Jan Čechal³, Josef Polčák³

¹ Central European Institute of Technology - CEITEC, Masaryk University, Brno, Czech Republic

² Faculty of Science, Masaryk University, Brno, Czech Republic

³ Central European Institute of Technology - CEITEC, Brno University of Technology, Brno, Czech Republic

lenkaz@physics.muni.cz

The bio-applications of amine thin films, such as a biomolecule immobilization, biosensors or a cell adhesion enhancement require good layer stability in water. However, an improved film stability achieved by increased polymer cross-linking results in lower density of amine groups for typical allylamine or ammonia/ethylene plasma polymerization process. Cyclopropylamine (CPA) is a promising non-toxic monomer recently used for the deposition of amine-rich thin films. A relatively good water stability reported for CPA plasma polymers was promising for the bio-applications but these studies were not carried out so far.

In this work, the electrospun polycaprolactone (PCL) micro/nanofibers with the mean thickness of 1.2 ± 0.4 µm and a pore size of 8 ± 2 µm and cell cultivation dishes were coated by amine-rich thin films using low pressure capacitively coupled plasma polymerization of CPA in continuous wave and pulsed mode. In pulsed discharges the film exhibited 7 at.% of amine groups and only 1 % of the thickness was lost after immersion of in water for 216 hours. The deposition of this coating improved the cell adhesion and proliferation, as the number of mouse myoblasts grown on the substrate surface during 1 day of cultivation increased by a factor of five after deposition of CPA plasma polymer compared to the uncoated substrate.

Additionally, CPA plasma polymerization was employed to deposit stable amine thin films on the surface of quartz crystal microbalance (QCM) biosensors. The antibody specific to human serum albumin (anti-HSA) was attached to the QCM surface via cross-linkage obtained by intermediate reaction with glutaraldehyde. All steps of the bio-immobilization were studied by X-ray photoelectron and infrared spectroscopy to characterize surface and layer chemistry. The immunosensor specificity towards the HSA antigen was confirmed by the flow test for three QCM sensors which preparation started with CPA plasma polymers of different thicknesses: 20, 100 and 350 nm. The strongest response to the HSA antigen was observed for the thinnest CPA plasma polymer film and the sensor sensitivity was in this case better than for the previously reported QCM sensors functionalized by the thiol-based self-assembled monolayer.